Rapid isolation method of soil bacilli and screening of their quorum quenching activity
نویسندگان
چکیده
In Gram-negative bacteria, quorum sensing is mediated by N-acylhomoserine lactones (AHL) to regulate different biological functions, including production of virulence factors. Quorum quenching refers to the interruption of quorum sensing, and the most attractive way is to degrade the AHL molecules. With the aim of isolating soil bacteria capable of blocking quorum sensing by inactivating AHL, a novel enrichment method has been designed. Bacteria degrading AHL were isolated from a Malaysian soil sample. We identified twelve isolates which possessed an aiiA homologue gene by using PCR approach and three strains were selected for further analysis. AHL-inactivation assay showed that these three isolates rapidly degraded synthetic 3-oxo-C6-HSL in vitro. Morphological phenotypes and 16S ribosomal DNA sequence analysis indicated that these isolates fell into the genus of Bacillus. Our data suggest that soil Bacillus spp. can be isolated using the selection method and a number of soil bacilli in Malaysian soil exhibited quorum quenching activity.
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